Chicken IL17(Interleukin 17) ELISA Kit

ELK Biotechnology
Product Code: ELK7652
Product Group: ELISA Kits
CodeSizePrice
ELK7652-48T48T£359.00
Quantity:
ELK7652-96T96T£477.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species: Chicken (Gallus)
Application: Enzyme-Linked Immunosorbent Assay (ELISA)

Video

Further Information

Alternative Names:
IL-17;IL17A; CTLA8; IL-17A; Cytotoxic T-Lymphocyte-Associated Protein 8
Assay Length:
2h
Assay Type:
Competitive Inhibition
Detection Range:
15.63-1000 pg/mL
Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
25.4 pg/mL
Standard:
1000 pg/mL
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken IL17 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken IL17. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Chicken IL17 in the samples is then determined by comparing the OD of the samples to the standard curve.