Human 8-iso prostaglandin F2α (8-iso-PGF2a) ELISA kit
| Code | Size | Price |
|---|
| ELK8402-48T | 48T | £298.00 |
Quantity:
| ELK8402-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
8-isoprostane; 8-iso-PGF2α
Assay Length:
2h
Assay Type:
Competitive Inhibition
Detection Range:
31.25-2000 pg/mL
Sample Type:
serum, plasma and other biological fluids
Sensitivity:
8.2 pg/mL
Standard:
2000 pg/mL
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 8-iso-PGF2a protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 8-iso-PGF2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human 8-iso-PGF2a in the samples is then determined by comparing the OD of the samples to the standard curve.