Human Ab1-42(Amyloid Beta Peptide 1-42) ELISA Kit
| Code | Size | Price |
|---|
| ELK2100-48T | 48T | £298.00 |
Quantity:
| ELK2100-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
Amyloid beta 42(ABeta 42); amyloid beta peptide 1-42; Aβ1-42,amyloid βpeptide 1-42
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
15.63-1000 pg/mL
Sample Type:
serum, plasma and other biological fluids
Sensitivity:
5.31 pg/mL
Standard:
1000 pg/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Ab1-42. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Ab1-42. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Ab1-42, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human Ab1-42 in the samples is then determined by comparing the OD of the samples to the standard curve.