Human AP17(Apelin 17) ELISA Kit
| Code | Size | Price |
|---|
| ELK4210-48T | 48T | £298.00 |
Quantity:
| ELK4210-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
APLN17
Assay Length:
2h
Assay Type:
Competitive Inhibition
Detection Range:
31.25-2000 pg/mL
Sample Type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
9.14 pg/mL
Standard:
2000 pg/mL
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AP17 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP17. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human AP17 in the samples is then determined by comparing the OD of the samples to the standard curve.