Human LAB7-1(B-Lymphocyte Activation Antigen B7-1) ELISA Kit
| Code | Size | Price |
|---|
| ELK5326-48T | 48T | £298.00 |
Quantity:
| ELK5326-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
CD80; CD28LG; CD28LG1; BB1; B7-1 Antigen; T-lymphocyte activation antigen CD80; CTLA-4 counter-receptor B7.1
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
78.13-5000 pg/mL
Sample Type:
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity:
33 pg/mL
Standard:
5000 pg/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LAB7-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LAB7-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LAB7-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human LAB7-1 in the samples is then determined by comparing the OD of the samples to the standard curve.