Human GT(Gastrin) ELISA Kit

ELK Biotechnology
Product Code: ELK1814
Product Group: ELISA Kits
CodeSizePrice
ELK1814-48T48T£298.00
Quantity:
ELK1814-96T96T£390.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)

Video

Further Information

Alternative Names:
GAST; GAS; Gastrin component I; Big gastrin; Gastrin component II; Gastrin component III; Gastrin 17; Gastrin 34
Assay Length:
2h
Assay Type:
Competitive Inhibition
Detection Range:
15.63-1000 pg/mL
Sample Type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
4.39 pg/mL
Standard:
1000 pg/mL
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human GT in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
P01350