Human IFNa/bR2(Interferon Alpha/Beta Receptor 2) ELISA Kit
| Code | Size | Price |
|---|
| ELK3549-48T | 48T | £298.00 |
Quantity:
| ELK3549-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
IFNαR2; IFN-alpha-REC; IFN-R; IFNαBR; IFNαRB; Interferon(Alpha,Beta And Omega)Receptor Beta Chain; Interferon alpha binding protein; Type I interferon receptor 2
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
0.63-40 ng/mL
Sample Type:
Serum, plasma, urine and other biological fluids.
Sensitivity:
0.262 ng/mL
Standard:
40 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IFNa/bR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IFNa/bR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IFNa/bR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human IFNa/bR2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
P48551