Human MTX(Methotrexate) ELISA Kit
| Code | Size | Price |
|---|
| ELK8649-48T | 48T | £261.00 |
Quantity:
| ELK8649-96T | 96T | £335.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
Methotrexate; Amethopterin
Assay Length:
2h
Assay Type:
Competitive Inhibition
Detection Range:
15.63-1000 ng/mL
Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
4.51 ng/mL
Standard:
1000 ng/mL
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MTX protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MTX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human MTX in the samples is then determined by comparing the OD of the samples to the standard curve.