Human ECE2(Endothelin Converting Enzyme 2) ELISA Kit
| Code | Size | Price |
|---|
| ELK5083-48T | 48T | £298.00 |
Quantity:
| ELK5083-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
Methyltransferase-like region
Assay Length:
2h
Assay Type:
Competitive Inhibition
Detection Range:
156.25-10000 pg/mL
Sample Type:
Tissue homogenates, cell lysates and other biological fluids.
Sensitivity:
45.9 pg/mL
Standard:
10000 pg/mL
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human ECE2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ECE2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human ECE2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
P0DPD6