Human IL12B(Interleukin 12B) ELISA Kit
| Code | Size | Price |
|---|
| ELK2263-48T | 48T | £298.00 |
Quantity:
| ELK2263-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
IL-12B; p40; IL12B; CLMF2; NKSF2; Natural Killer Cell Stimulatory Factor 2; Cytotoxic Lymphocyte Maturation Factor 2; Interleukin 23, P40 Subunit; Interleukin 12B
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
7.82-500 pg/mL
Sample Type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sensitivity:
2.8 pg/mL
Standard:
500 pg/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-12p40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL-12p40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL-12p40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human IL-12p40 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
P29460