Human P4Ha2(Prolyl-4-Hydroxylase Alpha Polypeptide II) ELISA Kit
| Code | Size | Price |
|---|
| ELK3814-48T | 48T | £298.00 |
Quantity:
| ELK3814-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
Procollagen-Proline;2-Oxoglutarate 4-Dioxygenase(Proline 4-Hydroxylase);Alpha Polypeptide II
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
0.16-10 ng/mL
Sample Type:
Tissue homogenates and other biological fluids.
Sensitivity:
0.063 ng/mL
Standard:
10 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human P4Ha2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human P4Ha2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human P4Ha2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human P4Ha2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
O15460