Human Nesp1(Nesprin 1) ELISA Kit

ELK Biotechnology
Product Code: ELK4139
Product Group: ELISA Kits
CodeSizePrice
ELK4139-48T48T£298.00
Quantity:
ELK4139-96T96T£390.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)

Video

Further Information

Alternative Names:
SYNE1; C6orf98; 8B; MYNE1; CPG2; SCAR8; ARCA1; Enaptin; Myocyte nuclear envelope protein 1; Nuclear envelope spectrin repeat protein 1; Synaptic nuclear envelope protein 1
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
0.32-20 ng/mL
Sample Type:
Tissue homogenates and other biological fluids.
Sensitivity:
0.105 ng/mL
Standard:
20 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Nesp1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Nesp1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Nesp1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human Nesp1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
Q8NF91