ELK Biotechnology

Human THRa(Thyroid Hormone Receptor Alpha) ELISA Kit

Product Code:
 
ELK3974
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK3974-48T48T£298.00
Quantity:
ELK3974-96T96T£390.00
Quantity:
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Further Information

Alternative Names:
THR-A; NR1A1; AR7; EAR7; ERB-T1; ERBA1; NR1-A1; THRA1; THRA2; c-ERBA1; Erythroblastic Leukemia Viral Oncogene Homolog; Nuclear Receptor Subfamily 1,Group A,Member 1
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
62.5-4000 pg/mL
Sample Type:
Tissue homogenates and other biological fluids.
Sensitivity:
22.5 pg/mL
Standard:
4000 pg/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human THRa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human THRa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human THRa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human THRa in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
P10827