Mouse a1M(Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit
| Code | Size | Price |
|---|
| ELK5551-48T | 48T | £298.00 |
Quantity:
| ELK5551-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Mouse
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
AMBP; UTI; HCP; EDC1; HI30; IATIL; ITILC; ITI; ITIL; Alpha 1 Microglobulin/Bikunin Precursor; Growth-inhibiting protein 19; Uristatin; Uronic-Acid-Rich Protein; Trypstatin
Assay Length:
2h
Assay Type:
Competitive Inhibition
Detection Range:
3.13-200 ug/mL
Sample Type:
serum, plasma and other biological fluids
Sensitivity:
0.94 ug/mL
Standard:
200 ug/mL
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse a1M protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse a1M. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Mouse a1M in the samples is then determined by comparing the OD of the samples to the standard curve.