Mouse C5aR1(Complement Component 5a Receptor 1) ELISA Kit
| Code | Size | Price |
|---|
| ELK4243-48T | 48T | £298.00 |
Quantity:
| ELK4243-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Mouse
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
CD88; C5A; C5-AR1; C5R1; C5a anaphylatoxin chemotactic receptor 1
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
0.32-20 ng/mL
Sample Type:
tissue homogenates, cell lysates and other biological fluids
Sensitivity:
0.115 ng/mL
Standard:
20 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse C5aR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse C5aR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse C5aR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Mouse C5aR1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
P30993