Mouse 20S-PSM(20S-Proteasome) ELISA Kit
| Code | Size | Price |
|---|
| ELK6913-48T | 48T | £298.00 |
Quantity:
| ELK6913-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Mouse
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
CP; 20SPSM; 20S Core Particle
Assay Length:
2h
Assay Type:
Competitive Inhibition
Detection Range:
468.75-30000 ng/mL
Sample Type:
Serum, plasma and other biological fluids.
Sensitivity:
152.4 ng/mL
Standard:
30000 ng/mL
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse 20S-PSM protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse 20S-PSM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Mouse 20S-PSM in the samples is then determined by comparing the OD of the samples to the standard curve.