Mouse NFkB(Nuclear Factor Kappa B) ELISA Kit
| Code | Size | Price |
|---|
| ELK1337-48T | 48T | £261.00 |
Quantity:
| ELK1337-96T | 96T | £335.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Mouse
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
NF-KB1; EBP-1; KBF1; NF-Kappa-B; NFKB-P105; NFKB-P50; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear factor NF-kappa-B p105 subunit; NFkB; Nuclear Factor Kappa B
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
0.16-10 ng/mL
Sample Type:
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
0.057 ng/mL
Standard:
10 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NF-κBp105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NF-κBp105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NF-κBp105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Mouse NF-κBp105 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
P25799