Mouse RELA(V-Rel Reticuloendotheliosis Viral Oncogene Homolog A) ELISA Kit
| Code | Size | Price |
|---|
| ELK8449-48T | 48T | £298.00 |
Quantity:
| ELK8449-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Mouse
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
NFKB3; p65; REL-A; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3; Transcription Factor P65; Nuclear factor NF-kappa-B p65 subunit; RELA; V-Rel Reticuloendotheliosis Viral Oncogene Homolog A
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
31.25-2000 pg/mL
Sample Type:
Tissue homogenates, cell lysates and other biological fluids
Sensitivity:
11.2 pg/mL
Standard:
2000 pg/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NFKB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NFKB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NFKB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Mouse NFKB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
Q04207