Mouse PAI2(Plasminogen Activator Inhibitor 2) ELISA Kit

ELK Biotechnology
Product Code: ELK2582
Product Group: ELISA Kits
CodeSizePrice
ELK2582-48T48T£298.00
Quantity:
ELK2582-96T96T£390.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species: Mouse
Application: Enzyme-Linked Immunosorbent Assay (ELISA)

Video

Further Information

Alternative Names:
SERPINB2; PLANH2; Serpin Peptidase Inhibitor Clade B Member 2; Monocyte Arg-serpin; Placental plasminogen activator inhibitor; Serpin B2; Urokinase inhibitor
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
7.82-500 pg/mL
Sample Type:
plasma
Sensitivity:
3.2 pg/mL
Standard:
500 pg/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PAI2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PAI2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PAI2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Mouse PAI2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
P12388