ELK Biotechnology

Mouse sRAGE(Soluble receptor for advanced glycation endproducts) ELISA Kit

Product Code:
 
ELK8357
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK8357-48T48T£298.00
Quantity:
ELK8357-96T96T£390.00
Quantity:
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This product comes from: China.
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Further Information

Alternative Names:
Soluble receptor for advanced glycation endproducts; sRAGE
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
2.6-165 pg/mL
Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
2.1 pg/mL
Standard:
165 pg/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse sRAGE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse sRAGE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse sRAGE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Mouse sRAGE in the samples is then determined by comparing the OD of the samples to the standard curve.