Mouse Lptn/LTN/XCL1 (Lymphotactin)ELISA Kit

ELK Biotechnology
Product Code: ELK8890
Product Group: ELISA Kits
CodeSizePrice
ELK8890-48T48T£359.00
Quantity:
ELK8890-96T96T£477.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species: Mouse
Application: Enzyme-Linked Immunosorbent Assay (ELISA)

Video

Further Information

Alternative Names:
Xcl1 ; Lptn ; Ltn ; Scyc1 ; Lymphotactin ; C motif chemokine 1 ; Cytokine SCM-1 ; Lymphotaxin ; Small-inducible cytokine C1
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
7.82-500 pg/ml
Sample Type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sensitivity:
3.27 pg/mL
Standard:
500 pg/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Lptn/LTN/XCL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Lptn/LTN/XCL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Lptn/LTN/XCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Mouse Lptn/LTN/XCL1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
P47993