Pig EDN1(Endothelin 1) ELISA Kit
| Code | Size | Price |
|---|
| ELK7427-48T | 48T | £359.00 |
Quantity:
| ELK7427-96T | 96T | £477.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Porcine (pig)
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
15.63-1000 pg/mL
Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
4.74 pg/mL
Standard:
1000 pg/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Pig ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
P09558