Pig F1+2(Prothrombin Fragment 1+2) ELISA Kit
| Code | Size | Price |
|---|
| ELK6049-48T | 48T | £359.00 |
Quantity:
| ELK6049-96T | 96T | £477.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Porcine (pig)
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
F1+2
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
6.25-400 ng/mL
Sample Type:
Serum, plasma and other biological fluids
Sensitivity:
2.52 ng/mL
Standard:
400 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig F1+2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig F1+2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig F1+2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Pig F1+2 in the samples is then determined by comparing the OD of the samples to the standard curve.