Rabbit C5a(Complement Component 5a) ELISA Kit
| Code | Size | Price |
|---|
| ELK3035-48T | 48T | £359.00 |
Quantity:
| ELK3035-96T | 96T | £477.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Rabbit
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
Complement Component 5a
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
6.25-400 pg/mL
Sample Type:
Serum, plasma and other biological fluids
Sensitivity:
2.82 pg/mL
Standard:
400 pg/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit C5a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit C5a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit C5a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rabbit C5a in the samples is then determined by comparing the OD of the samples to the standard curve.