Rabbit Cr(Calretinin) ELISA Kit
Code | Size | Price |
---|
ELK9056-48T | 48T | £298.00 |
Quantity:
ELK9056-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Rabbit
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
CR; Calretinin; CALB2; CAB29; CAL2; 29 kDa calbindin; calbindin 2; calbindin 2; ; 29kD; calretinin; calbindin D29K
Assay Length:
2h
Assay Type:
Competitive Inhibition
Detection Range:
1.25-80 nmol/ml
Sample Type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sensitivity:
0.75 nmol/ml
Standard:
80 nmol/ml
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rabbit CR protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit CR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rabbit CR in the samples is then determined by comparing the OD of the samples to the standard curve.