Rat ANP(Atrial Natriuretic Peptide) ELISA Kit

ELK Biotechnology
Product Code: ELK5450
Product Group: ELISA Kits
CodeSizePrice
ELK5450-48T48T£298.00
Quantity:
ELK5450-96T96T£390.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species: Rat
Application: Enzyme-Linked Immunosorbent Assay (ELISA)

Video

Further Information

Alternative Names:
ANH; ANF; CDD; Atrial Natriuretic Factor; Atrial Natriuretic Hormone; Atriopeptin; Cardionatrine; Cardiodilatin
Assay Length:
2h
Assay Type:
Competitive Inhibition
Detection Range:
15.63-1000 pg/mL
Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
5.11 pg/mL
Standard:
1000 pg/mL
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat ANP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat ANP in the samples is then determined by comparing the OD of the samples to the standard curve.