Rat b2M(Beta-2-Microglobulin) ELISA Kit
| Code | Size | Price |
|---|
| ELK4881-48T | 48T | £298.00 |
Quantity:
| ELK4881-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Rat
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
Beta 2M; BMG; B2MG; b2M; Beta-2-Microglobulin
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
0.32-20 ng/mL
Sample Type:
serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
0.134 ng/mL
Standard:
20 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat BMG/b2-MG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat BMG/b2-MG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat BMG/b2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat BMG/b2-MG in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
P07151