Rat TPO-Ab(anti-Thyroid-Peroxidase antibody) ELISA Kit
| Code | Size | Price |
|---|
| ELK8352-48T | 48T | £298.00 |
Quantity:
| ELK8352-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Rat
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
TPO Ab; TPO-Ab
Assay Length:
2h
Assay Type:
Competitive Inhibition
Detection Range:
1.25-80 IU/mL
Sample Type:
serum, plasma, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
1.25 IU/mL
Standard:
80 IU/mL
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat TPO-Ab protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TPO-Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat TPO-Ab in the samples is then determined by comparing the OD of the samples to the standard curve.