Rat THBS1(Thrombospondin 1) ELISA Kit
| Code | Size | Price |
|---|
| ELK1686-48T | 48T | £298.00 |
Quantity:
| ELK1686-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Rat
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
TSP1; Thrombospondin-1p180; THBS1; Thrombospondin 1
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
7.82-500 pg/mL
Sample Type:
Plasma
Sensitivity:
3.6 pg/mL
Standard:
500 pg/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TSP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TSP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TSP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat TSP-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
Q71SA3