Simian CA2(Carbonic Anhydrase II) ELISA Kit

ELK Biotechnology
Product Code: ELK6774
Product Group: ELISA Kits
CodeSizePrice
ELK6774-48T48T£359.00
Quantity:
ELK6774-96T96T£477.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species: Simian
Application: Enzyme-Linked Immunosorbent Assay (ELISA)

Video

Further Information

Alternative Names:
CA-II; CAII; Car2; CAC; Carbonate dehydratase II; Carbonic anhydrase C
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
3.13-200 ng/mL
Sample Type:
Serum, plasma and other biological fluids.
Sensitivity:
1.22 ng/mL
Standard:
200 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian CA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian CA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian CA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Simian CA2 in the samples is then determined by comparing the OD of the samples to the standard curve.