Dog IP10(Interferon Gamma Induced Protein 10kDa) ELISA Kit
| Code | Size | Price |
|---|
| ELK5805-48T | 48T | £359.00 |
Quantity:
| ELK5805-96T | 96T | £477.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Canine (dog)
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
CXCL10; C7; IFI10; INP10; SCYB10; Crg2; GIP10; Mob1; Chemokine C-X-C Motif Ligand 10; Small Inducible Cytokine Subfamily B(Cys-X-Cys)Member 10
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
15.63-1000 pg/mL
Sample Type:
serum, plasma and other biological fluids
Sensitivity:
5.9 pg/mL
Standard:
1000 pg/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog IP10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog IP10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog IP10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Dog IP10 in the samples is then determined by comparing the OD of the samples to the standard curve.