Dog BNP(Brain Natriuretic Peptide) ELISA Kit

ELK Biotechnology
Product Code: ELK7250
Product Group: ELISA Kits
CodeSizePrice
ELK7250-48T48T£359.00
Quantity:
ELK7250-96T96T£477.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species: Canine (dog)
Application: Enzyme-Linked Immunosorbent Assay (ELISA)

Video

Further Information

Alternative Names:
GC-B; B-Type Natriuretic Peptide; Ventricular Natriuretic Peptide; Gamma-brain natriuretic peptide; Brain natriuretic peptide 32
Assay Length:
2h
Assay Type:
Competitive Inhibition
Detection Range:
31.25-2000 pg/mL
Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
9.55 pg/mL
Standard:
2000 pg/mL
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog BNP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog BNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Dog BNP in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
P16859