Cattle TGFb1(Transforming Growth Factor Beta 1) ELISA Kit

ELK Biotechnology
Product Code: ELK5467
Product Group: ELISA Kits
CodeSizePrice
ELK5467-48T48T£359.00
Quantity:
ELK5467-96T96T£477.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species: Bovine (Cattle)
Application: Enzyme-Linked Immunosorbent Assay (ELISA)

Video

Further Information

Alternative Names:
TGF Beta 1; TGF-B1; CED; DPD1; LAP; Camurati-Engelmann Disease; Latency-associated peptide
Assay Length:
2h
Assay Type:
Competitive Inhibition
Detection Range:
15.63-1000 pg/mL
Sample Type:
serum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids
Sensitivity:
4.67 pg/mL
Standard:
1000 pg/mL
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle TGFb1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle TGFb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Cattle TGFb1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
P18341