General Hyp(Hydroxyproline) ELISA Kit
| Code | Size | Price |
|---|
| ELK7835-48T | 48T | £261.00 |
Quantity:
| ELK7835-96T | 96T | £335.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Species Independent
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
(2S,4R)-4-Hydroxyproline; L-Hydroxyproline(C5H9O3N)
Assay Length:
2h
Assay Type:
Competitive Inhibition
Detection Range:
78.13-5000 ng/mL
Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
26.3 ng/mL
Standard:
5000 ng/mL
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Hyp protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hyp. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Hyp in the samples is then determined by comparing the OD of the samples to the standard curve.