General Biotin ELISA Kit
| Code | Size | Price |
|---|
| ELK8601-48T | 48T | £359.00 |
Quantity:
| ELK8601-96T | 96T | £477.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Species Independent
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
Biotin
Assay Length:
2h
Assay Type:
Competitive Inhibition
Detection Range:
3.13-200 pg/mL
Sample Type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sensitivity:
4.7 pg/mL
Standard:
200 pg/mL
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Biotin protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Biotin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Biotin in the samples is then determined by comparing the OD of the samples to the standard curve.