High Sensitivity Human PTH(Parathyroid Hormone) ELISA Kit
| Code | Size | Price |
|---|
| ELK8878-48T | 48T | £359.00 |
Quantity:
| ELK8878-96T | 96T | £477.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin; PTH; Parathyroid Hormone
Assay Length:
2h
Assay Type:
Competitive Inhibition
Detection Range:
3.13-200 pg/mL
Sample Type:
serum, plasma and other biological fluids
Sensitivity:
0.9 pg/mL
Standard:
200 pg/mL
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human High Sensitivity? I-PTH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human High Sensitivity? I-PTH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human High Sensitivity? I-PTH in the samples is then determined by comparing the OD of the samples to the standard curve.