SARS-CoV-2 (COVID-19) Spike P681R Antibody [7E3C5] (Delta Variant)

Product Code: PSI-PM-9680

Supplier: ProSci

Code	Size	Price

PSI-PM-9680-0.02mg

0.02mg

£150.00

Quantity:

PSI-PM-9680-0.1mg

0.1mg

£515.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Mouse

Antibody Isotype: IgG2

Antibody Clonality: Monoclonal

Antibody Clone: 7E3C5

Regulatory Status: RUO

Target Species: SARS-CoV-2

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Immunohistochemistry- Paraffin Embedded (IHC-P)

Storage:

SARS-CoV-2 Spike P681R (Delta Variant) antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 Immunohistochemistry Validation of Spike in Delta Variant COVID-19 Patient Lung Tissue</strong><br>
Immunohistochemical analysis of paraffin-embedded patient lung tissue using anti-Spike P681R antibody (PM-9680) at 20 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. <strong>Strong spike protein signal was observed in the Delta variant COVID-19 patient lung, but not in WT COVID-19 patient lung. </strong>

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<strong>Figure 2 SARS-Cov-2 Spike P681R (Delta Variant) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISA </strong><br>
Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 μg/mL, incubated at 4 ˚C overnight.
Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, PM-9680, 1 μg/mL, incubated at RT for 1 hr.
Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.

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<strong>Figure 3 ELISA Validation of Delta Variant Spike S1 Antibodies with SARS-CoV-2 Delta Variant Spike S1 Protein </strong><br>
Coating Antigen: SARS-CoV-2 spike S1 proteins, WT, Alpha and Delta variants, 1 μg/mL, incubated at 4 ˚C overnight.
Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, PM-9680, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.
Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.
<strong>SARS-CoV-2 Spike P681R (Delta Variant) antibody specifically detects spike S1 protein of Delta variant, but not WT and Alpha variant. </strong>

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<strong>Figure 4 ELISA Validation of Delta Variant Spike S1 Antibodies with Mutant and WT Peptide </strong><br>
Coating Antigen: SARS-CoV-2 spike S1 peptides: WT (9091P), Alpha variant (9359P), and Delta variant (9673P), 1 μg/mL, incubated at 4 ˚C overnight.
Detection Antibodies: SARS-CoV-2 delta spike P681R antibody, PM-9680, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.
Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.
<strong>SARS-CoV-2 Spike P681R (Delta Variant) antibody detects Delta variant spike S1 peptide (681R, 9673P), but not WT peptide (681P, 9091P) and Alpha variant spike S1 peptide (681H, 9359P). </strong>

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Documents

Further Information

Additional Names:

Anti-SARS-CoV-2 Spike P681R (Delta Variant) antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein, Delta variant

Application Note:

Anti-SARS-CoV-2 Spike P681R (Delta Variant) antibody specifically detects SARS-CoV-2 Delta Variant Spike S1 protein, but not SARS-CoV-2 WT and other variant Spike S1 protein by ELISA. All other applications and species not yet tested.

Background:

SARS-CoV-2 delta variant, a variant of concern (VOC), known as B.1.617.2, was detected in India in October of 2020. However, it rapidly spread all over of the world and now it is the dominant variant in the world, which account for more than 99% of the cases. This variant carries at least 13 mutations in spike protein across the sub lineages, including L452R, D614G, P681R and K417N, which can increase the affinity to the human ACE2 receptor. Enhanced transmission of the Delta variant was observed globally, which is at least 2.5 times more contagious as the other variants. The Delta variant affects the effectiveness of COVID19 vaccine and is resistant to neutralization to some extent.

Background References:

Zhang et al. JAMA 2021, 325 (13): 1324-26.
Reardon. Nature 2021.
Delphine. Nature 2021, 596 (7871): 276-280.

Buffer:

SARS-CoV-2 Spike P681R (Delta Variant) antibody is supplied in PBS containing 0.02% sodium azide.

Concentration:

1 mg/mL

Conjugate:

Unconjugated

Homology:

Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (25%)

Immunogen:

Anti-SARS-CoV-2 Spike 681R (Delta Variant) antibody (PM-9680) was raised against a peptide corresponding to 12 amino acids near the cleavage site of SARS-CoV-2 Delta variant Spike protein.

The immunogen is located within 650-700 amino acids of SARS-CoV-2 Delta variant Spike protein.

NCBI Gene ID #:

43740568

NCBI Official Name:

surface glycoprotein

NCBI Official Symbol:

NCBI Organism:

Severe acute respiratory syndrome coronavirus 2

Physical State:

Liquid

Protein Accession #:

QHD43416

Protein GI Number:

1791269090

Purification:

Anti-SARS-CoV-2 Spike P681R (Delta Variant) antibody is Protein G purified.

Research Area:

Infectious Disease,COVID-19

SPECIFICITY:

It can only detect SARS-CoV-2 Delta Variant Spike S1 protein and does not cross-react with the spike protein of other variants.

Swissprot #:

P0DTC2

User NOte:

Optimal dilutions for each application to be determined by the researcher.