SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [75G5a]

Contact us
ProSci
Product Code: PSI-10-750
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-10-750-0.1mg0.1mg£657.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG1k
Antibody Clonality: Monoclonal
Antibody Clone: 75G5a
Regulatory Status: RUO
Target Species: SARS-CoV-2
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
blue ice or RT
Storage:
SARS-CoV-2 (COVID-19) Nucleoprotein Antibody [75G5a] can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 5
<strong>Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Nucleocapsid in COVID-19 Patient Lung</strong><br> Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Nucleocapsid antibody (10-750, 1 ng/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Nucleocapsid protein was observed in the macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.
2 / 5
3 / 5
A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies: 75G5a as a capture antibody and 85C10-biotin as a detection antibody. COVID-19 NP was serially diluted 1:2 starting at 20 ng/mL. Rabbit monoclonal antibodies 75G5a and 85C10-biotin detected COVID-19 NP antigen at very high sensitivity as low as 19.5 pg/mL (1.9 pg).
4 / 5
A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies: 75G5a as a capture antibody and 85C1-biotin as a detection antibody. COVID-19 NP was serially diluted 1:2 starting at 25 ng/mL. Rabbit monoclonal antibodies 75G5a and 85C1-biotin detected COVID-19 NP antigen at very high sensitivity as low as 100 pg/mL (10 pg).
5 / 5
Western blot analysis of SARS-CoV2 and SARS-CoV nucleocapsid protein (50 ng) probed with 1 ug/mL CoV2NP rabbit monoclonal Antibody (75G5a), CoV2NP-75G5.
❮❯

<strong>Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Nucleocapsid in COVID-19 Patient Lung</strong><br> Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Nucleocapsid antibody (10-750, 1 ng/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Nucleocapsid protein was observed in the macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.
A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies: 75G5a as a capture antibody and 85C10-biotin as a detection antibody. COVID-19 NP was serially diluted 1:2 starting at 20 ng/mL. Rabbit monoclonal antibodies 75G5a and 85C10-biotin detected COVID-19 NP antigen at very high sensitivity as low as 19.5 pg/mL (1.9 pg).
A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies: 75G5a as a capture antibody and 85C1-biotin as a detection antibody. COVID-19 NP was serially diluted 1:2 starting at 25 ng/mL. Rabbit monoclonal antibodies 75G5a and 85C1-biotin detected COVID-19 NP antigen at very high sensitivity as low as 100 pg/mL (10 pg).
Western blot analysis of SARS-CoV2 and SARS-CoV nucleocapsid protein (50 ng) probed with 1 ug/mL CoV2NP rabbit monoclonal Antibody (75G5a), CoV2NP-75G5.

Documents

Further Information

Additional Names:
SARS-CoV-2 (COVID-19) Nucleoprotein Antibody [P001A]: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Application Note:
Paired with 84C4a or 84D7, used for specific, high-sensitivity detection of SARS-CoV-2 nucleocapsid protein (NP).
Background:
We have leveraged our next-generation rabbit mAb discovery platform to develop recombinant rabbit monoclonal antibodies with extremely high sensitivity and specificity to SARS-CoV-2. These antibodies, which include clones P001A, P002A, and P003A can be paired in sandwich detection assay and used to detect nucleoprotein (NP) antigen from SARS-CoV-2. These antibodies do not cross-react to the highly related SARS-CoV virus or to any other coronaviruses tested. Sandwich ELISA detection using TMB/acid developer reliably detects NP antigen in the pg/mL range, and sensitivity is expected to be even higher when using more sensitive developer strategies. This panel of antibodies can be used as raw materials for diagnostic kits and can be applied to lateral flow systems for diagnostic detection of COVID-19.
Buffer:
1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Highly-specific to SARS-CoV-2 nucleoprotein; does not cross-react with nucleoprotein from SARS-CoV or other coronaviruses
Immunogen:
SARS-CoV-2 nucleoprotein (NP) specific peptide
NCBI Gene ID #:
43740575
NCBI Official Name:
Nucleocapsid Phosphoprotein
NCBI Official Symbol:
N
NCBI Organism:
SARS-CoV-2
Physical State:
Liquid
Protein Accession #:
QHD43423
Protein GI Number:
1820552696
Purification:
SARS-CoV-2 (COVID-19) Nucleoprotein Antibody [75G5a] is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease,COVID-19
User NOte:
Optimal dilutions for each application to be determined by the researcher.