AIFM2 (CT) Antibody
Code | Size | Price |
---|
PSI-9653-0.02mg | 0.02mg | £150.00 |
Quantity:
PSI-9653-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
AIMF2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Further Information
Additional Names:
AIMF2
Application Note:
WB: 2 μg/mL; IHC: 1 μg/mL; IF: 20 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human samples and Immunohistochemistry in human mouse and rat samples. All other applications and species not yet tested.
Background:
AIFM2 Antibody: AIFM2 blocks ferroptosis independent of ubiquinol metabolism. AIFM2 is a key component of a non-mitochondrial CoQ antioxidant system that acts in parallel to the canonical glutathione-based GPX4 pathway. AIFM2 confers protection against ferroptosis elicited by GPX4 deletion. AIF-2 was found to be upregulated in human glioma tissues and cell lines. Downregulation of AIF-2 reduced cell proliferation and induced G1 cell cycle arrest. AIF and its family member protein, AMID, are rotenone-sensitive NADH:ubiquinone oxidoreductases (of the NDH-2 type). AIF-M2 lessens survival cell signaling in the presence of foreign (e.g. bacterial and (retro)viral) cytosolic DNA, thus contributing to the onset of apoptosis
Background References:
- Dai et al. Biochem Biophys Res Commun. 2020; 523(4):966-971
- Bersuker et al. Nature 2019; 575(7784):688-692
- Doll et al. Nature 2019; 575(7784):693-698
- Chen et al. J Mol Neurosci 2019; 68(2): 304-310
- Gong et al. J Biol Chem 2007; 282(41):30331-30340
Buffer:
AIFM2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
Homology:
Predicted species reactivity based on immunogen sequence: Gorilla (100%); chimpanzee (100%), cow (92%), Horse (92%)
Immunogen:
Anti-AIFM2 antibody ( 9653) was raised against a peptide corresponding to 12 amino acids near the carboxyl terminus of human AIFM2. The immunogen is located within the last 50 amino acids of AIFM2.
ISOFORMS:
Human AIFM2 has 2 isoforms, including isoform 1 (373aa, 40.5kD) and isoform 2 (336aa, 36.5kD). Mouse AIFM2 has 2 isoforms, isoform 1 (373aa, 40.6kD) and isoform 2 (380aa, 41.4kD). Rat AIFM2 has one isoform (373aa, 40.7kD). 9653 can detect AIFM2 in human, mouse and rat.
NCBI Gene ID #:
84883
NCBI Official Name:
apoptosis inducing factor mitochondria associated
NCBI Official Symbol:
AIMF2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 41kD
Observed: 41 kD
Observed: 41 kD
Protein Accession #:
Q9BRQ8
Protein GI Number:
74752283
Purification:
AIMF2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
Q9BRQ8
User NOte:
Optimal dilutions for each application to be determined by the researcher.