AIFM2 (IN) Antibody

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ProSci
Product Code: PSI-9655
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-9655-0.02mg0.02mg£150.00
Quantity:
PSI-9655-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
AIMF2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1: WB Validation in Human Mouse and Rat Cell Lines</strong><br> Loading: 15 μg of lysate. Antibodies: AIFM2 9655, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10,000 dilution.
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<strong>Figure 2: WB Validation in Mouse Skeletal Muscle</strong><br> Loading: 15 μg of lysate. Antibodies: AIFM2 9655, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10,000 dilution.
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<strong>Figure 3: WB Validation in Rat Tissues</strong><br> Loading: 15 μg of lysate. Antibodies: AIFM2 9655, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10,000 dilution.
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<strong>Figure 4: Immunofluorescence Validation of AIFM2 in HeLa Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling AIFM2 with 9655 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 5: Immunohistochemistry Validation of AIFM2 in Human Brain</strong><br> Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-AIFM2 antibody (9655) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 6: Immunohistochemistry Validation of AIFM2 in Mouse Brain</strong><br> Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-AIFM2 antibody (9655) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 7: Immunohistochemistry Validation of AIFM2 in Rat Brain</strong><br> Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-AIFM2 antibody (9655) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 1: WB Validation in Human Mouse and Rat Cell Lines</strong><br> Loading: 15 μg of lysate. Antibodies: AIFM2 9655, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10,000 dilution.
<strong>Figure 2: WB Validation in Mouse Skeletal Muscle</strong><br> Loading: 15 μg of lysate. Antibodies: AIFM2 9655, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10,000 dilution.
<strong>Figure 3: WB Validation in Rat Tissues</strong><br> Loading: 15 μg of lysate. Antibodies: AIFM2 9655, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10,000 dilution.
<strong>Figure 4: Immunofluorescence Validation of AIFM2 in HeLa Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling AIFM2 with 9655 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 5: Immunohistochemistry Validation of AIFM2 in Human Brain</strong><br> Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-AIFM2 antibody (9655) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 6: Immunohistochemistry Validation of AIFM2 in Mouse Brain</strong><br> Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-AIFM2 antibody (9655) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 7: Immunohistochemistry Validation of AIFM2 in Rat Brain</strong><br> Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-AIFM2 antibody (9655) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Documents

Further Information

Additional Names:
AIMF2
Application Note:
WB: 2 μg/mL; IHC: 1 μg/mL; IF: 20 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human samples and Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.
Background:
AIFM2 Antibody: AIFM2 blocks ferroptosis independent of ubiquinol metabolism. AIFM2 is a key component of a non-mitochondrial CoQ antioxidant system that acts in parallel to the canonical glutathione-based GPX4 pathway. AIFM2 confers protection against ferroptosis elicited by GPX4 deletion. AIF-2 was found to be upregulated in human glioma tissues and cell lines. Downregulation of AIF-2 reduced cell proliferation and induced G1 cell cycle arrest. AIF and its family member protein, AMID, are rotenone-sensitive NADH:ubiquinone oxidoreductases (of the NDH-2 type). AIF-M2 lessens survival cell signaling in the presence of foreign (e.g. bacterial and (retro)viral) cytosolic DNA, thus contributing to the onset of apoptosis
Background References:
  • Dai et al. Biochem Biophys Res Commun. 2020; 523(4):966-971
  • Bersuker et al. Nature 2019; 575(7784):688-692
  • Doll et al. Nature 2019; 575(7784):693-698
  • Chen et al. J Mol Neurosci 2019; 68(2): 304-310
  • Gong et al. J Biol Chem 2007; 282(41):30331-30340
Buffer:
AIFM2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
Homology:
Predicted species reactivity based on immunogen sequence: Gorilla (100%); chimpanzee (100%), cow (100%), Horse (93%)
Immunogen:
Anti-AIFM2 antibody ( 9655) was raised against a peptide corresponding to 14 amino acids near the center of human AIFM2. The immunogen is located within 130-180 amino acids of AIFM2.
ISOFORMS:
Human AIFM2 has 2 isoforms, including isoform 1 (373aa, 40.5kD) and isoform 2 (336aa, 36.5kD). Mouse AIFM2 has 2 isoforms, isoform 1 (373aa, 40.6kD) and isoform 2 (380aa, 41.4kD). Rat AIFM2 has one isoform (373aa, 40.7kD). 9653 can detect AIFM2 in human, mouse and rat.
NCBI Gene ID #:
84883
NCBI Official Name:
apoptosis inducing factor mitochondria associated
NCBI Official Symbol:
AIMF2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 41kD

Observed: 40 kD
Protein Accession #:
Q9BRQ8
Protein GI Number:
74752283
Purification:
AIFM2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
Q9BRQ8
User NOte:
Optimal dilutions for each application to be determined by the researcher.