NCOA4 (IN) Antibody

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ProSci
Product Code: PSI-9663
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-9663-0.02mg0.02mg£150.00
Quantity:
PSI-9663-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
NCOA4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1: Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: NCOA4 9663, 0.5 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
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<strong>Figure 2: Western Blot Validation in Mouse Tissues </strong><br> Loading: 15 μg of lysates per lane. Antibodies: NCOA4 9663, 1 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
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<strong>Figure 3: Western Blot Validation in Rat Tissues</strong><br> Loading: 15 μg of lysates per lane. Antibodies: NCOA4 9663, 1 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
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<strong>Figure 4: Immunofluorescence Validation of NCOA4 in Daudi Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling NCOA4 with 9663 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 5: Immunohistochemistry Validation of NCOA4 in Human lung</strong><br> Immunohistochemical analysis of paraffin-embedded human mouse tissue using anti-NCOA4 antibody (9663) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 6: Immunohistochemistry Validation of NCOA4 in Mouse lung</strong><br> Immunohistochemical analysis of paraffin-embedded mouse tissue using anti-NCOA4 antibody (9663) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 7: Immunohistochemistry Validation of NCOA4 in Rat lung</strong><br> Immunohistochemical analysis of paraffin-embedded rat tissue using anti-NCOA4 antibody (9663) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 1: Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: NCOA4 9663, 0.5 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 2: Western Blot Validation in Mouse Tissues </strong><br> Loading: 15 μg of lysates per lane. Antibodies: NCOA4 9663, 1 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 3: Western Blot Validation in Rat Tissues</strong><br> Loading: 15 μg of lysates per lane. Antibodies: NCOA4 9663, 1 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 4: Immunofluorescence Validation of NCOA4 in Daudi Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling NCOA4 with 9663 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 5: Immunohistochemistry Validation of NCOA4 in Human lung</strong><br> Immunohistochemical analysis of paraffin-embedded human mouse tissue using anti-NCOA4 antibody (9663) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 6: Immunohistochemistry Validation of NCOA4 in Mouse lung</strong><br> Immunohistochemical analysis of paraffin-embedded mouse tissue using anti-NCOA4 antibody (9663) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 7: Immunohistochemistry Validation of NCOA4 in Rat lung</strong><br> Immunohistochemical analysis of paraffin-embedded rat tissue using anti-NCOA4 antibody (9663) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Documents

Further Information

Additional Names:
NCOA4 Antibody: Nuclear receptor coactivator 4, NCoA-4, Androgen receptor coactivator 70 kDa protein, 70 kDa AR-activator, 70 kDa androgen receptor coactivator, Androgen receptor-associated protein of 70 kDa, Ret-activating protein ELE1, ARA70, ELE1, RFG
Application Note:
WB: 0.5-1 μg/mL; IHC: 1 μg/mL; IF: 20 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human samples and Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.
Background:
NCOA4 Antibody: Study showed that NCOA4-mediated ferritin complex degradation supplies iron to mitochondria. Loss of COPZ1 induces NCOA4 mediated autophagy and ferroptosis in glioblastoma cell lines. NCOA4-mediated ferritinophagy promoted inflammatory responses in periodontitis. Low expression of ferritinophagy-related NCOA4 gene in relation to unfavorable outcome and defective immune cells infiltration in clear cell renal carcinoma.
Background References:
  • Fujimaki et al. Mol Cell Biol. 2019; 39(14): e00010-19
  • Zhang et al. Oncogene. 2021; 40(8): 1425-1439
  • Guo et al. J Periodontal Res. 2021; 56(3): 523-534
  • Mou et al. BMC Cancer. 2021; 21(1):18
Buffer:
NCOA4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
Homology:
Predicted species reactivity based on immunogen sequence: monkey (100%), gorilla (100%), dog (100%), horse (100%)
Immunogen:
Anti-NCOA4 antibody (9663) was raised against a peptide corresponding to 13 amino acids near the center of human NCOA4. The immunogen is located within 420-470 amino acids of NCOA4.
ISOFORMS:
Human NCOA4 has 4 isoforms, including isoform alpha (614aa, 69.7kD), isoform beta (287aa, 32.6kD), isoform 3 (630aa, 71.4kD) and isoform 4 (650aa, 73.6kD). Mouse NCOA4 has at least one isoform (625aa, 70.4kD). Rat NCOA4 has one isoform (622aa, 70.3kD). 9663 can detect NCOA4 in human, mouse and rat.
NCBI Gene ID #:
8031
NCBI Official Name:
nuclear receptor coactivator 4
NCBI Official Symbol:
NCOA4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 70-74 kD

Observed: 70-74 kD
Protein Accession #:
Q13772
Protein GI Number:
8928226
Purification:
NCOA4 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
Q13772
User NOte:
Optimal dilutions for each application to be determined by the researcher.