PEX3 (CT) Antibody

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ProSci
Product Code: PSI-9665
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-9665-0.02mg0.02mg£150.00
Quantity:
PSI-9665-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
PEX3 antibody can be stored at 4 ˚C for three months and -20 ˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 WB Validation in Human Cell Lines </strong><br> Loading: 15 μg of lysate Antibodies: PEX3 9665, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
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<strong>Figure 2 Western Blot Validation in Mouse Tissues </strong><br> Loading: 15 μg of lysates per lane. Antibodies: PEX3 9665, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
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<strong>Figure 3 Western Blot Validation in Rat Tissues</strong><br> Loading: 15 μg of lysates per lane. Antibodies: PEX3 9665, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
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<strong>Figure 4 Immunofluorescence Validation of PEX3 in Human HeLa Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling PEX3 with 9665 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 5 Immunofluorescence Validation of PEX3 in Human Spleen</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling PEX3 with 9665 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 6 Immunofluorescence Validation of PEX3 in Mouse Thymus</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus labeling PEX3 with 9665 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 7 Immunofluorescence Validation of PEX3 in Rat testis</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PEX3 with 9665 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 1 WB Validation in Human Cell Lines </strong><br> Loading: 15 μg of lysate Antibodies: PEX3 9665, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 2 Western Blot Validation in Mouse Tissues </strong><br> Loading: 15 μg of lysates per lane. Antibodies: PEX3 9665, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 3 Western Blot Validation in Rat Tissues</strong><br> Loading: 15 μg of lysates per lane. Antibodies: PEX3 9665, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 4 Immunofluorescence Validation of PEX3 in Human HeLa Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling PEX3 with 9665 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 5 Immunofluorescence Validation of PEX3 in Human Spleen</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling PEX3 with 9665 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 6 Immunofluorescence Validation of PEX3 in Mouse Thymus</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus labeling PEX3 with 9665 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 7 Immunofluorescence Validation of PEX3 in Rat testis</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PEX3 with 9665 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Documents

Further Information

Additional Names:
PEX3 Antibody: Peroxisomal biogenesis factor 3, Peroxin-3, Peroxisomal assembly protein PEX3
Application Note:
WB: 2 μg/mL; IF: 20 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
Background:
PEX3 Antibody: PEX3 is involved in peroxisome biosynthesis and integrity. It assembles membrane vesicles before the matrix proteins are translocated. As a docking factor for PEX19, PEX3 is necessary for the import of peroxisomal membrane proteins in the peroxisomes. Thus within the cell, PEX3 is stabilized by PEX19 preventing PEX3 aggregation. Mutations in the PEX19-binding domain of PEX3 reduce the affinity for PEX19 and destabilize PEX3.
Background References:
  • Ghaedi et al. Mol Biol Cell. 2000; 11(6):2085-2102
  • Fang et al. J Cell Biol. 2004;164(6):863-875
  • Hattula et al. PloS One. 2014; 9(7):e103101
  • Schmidt et al. Traffic. 2012; 13(9):1244-1260
Buffer:
PEX3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
Homology:
Predicted species reactivity based on immunogen sequence: monkey (100%), cow (100%)
Immunogen:
Anti-PEX3 antibody (9665) was raised against a peptide corresponding to 12 amino acids near the carboxyl terminal of human PEX3. The immunogen is located within last 50 amino acids of PEX3.
ISOFORMS:
Human PEX3 has 1 isoform, including isoform 1 (373aa, 42kD). Mouse PEX3 has 1 isoform (372aa, 42kD). Rat PEX3 has one isoform (372aa, 42kD). 9665 can detect PEX3 in human, mouse and rat.
NCBI Gene ID #:
8504
NCBI Official Symbol:
PEX3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 42kD

Observed: 42 kD
Protein Accession #:
P56589
Protein GI Number:
3914303
Purification:
PEX3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
P56589
User NOte:
Optimal dilutions for each application to be determined by the researcher.