PEX10 (CT) Antibody

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ProSci
Product Code: PSI-9671
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-9671-0.02mg0.02mg£150.00
Quantity:
PSI-9671-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
PEX10 antibody can be stored at 4 ˚C for three months and -20 ˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 WB Validation in Human and Mouse Cell Lines</strong><br> Loading: 15 μg of lysate Antibodies: PEX10 9671, 4 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
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<strong>Figure 2 WB Validation in Mouse Tissues</strong><br> Loading: 15 μg of lysates per lane. Antibodies: PEX10 9671, 4 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
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<strong>Figure 3 WB Validation in Rat Tissues</strong><br> Loading: 15 μg of lysates per lane. Antibodies: PEX10 9671, 4 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
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<strong>Figure 4 Immunofluorescence Validation of PEX10 in Human Daudi Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling PEX10 with 9671 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 5 Immunofluorescence Validation of PEX10 in Human Spleen </strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling PEX10 with 9671 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 6 Immunofluorescence Validation of PEX10 in Mouse Thymus</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus labeling PEX10 with 9671 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 7 Immunofluorescence Validation of PEX10 in Rat Testis</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PEX10 with 9671 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 1 WB Validation in Human and Mouse Cell Lines</strong><br> Loading: 15 μg of lysate Antibodies: PEX10 9671, 4 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 2 WB Validation in Mouse Tissues</strong><br> Loading: 15 μg of lysates per lane. Antibodies: PEX10 9671, 4 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 3 WB Validation in Rat Tissues</strong><br> Loading: 15 μg of lysates per lane. Antibodies: PEX10 9671, 4 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 4 Immunofluorescence Validation of PEX10 in Human Daudi Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling PEX10 with 9671 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 5 Immunofluorescence Validation of PEX10 in Human Spleen </strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling PEX10 with 9671 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 6 Immunofluorescence Validation of PEX10 in Mouse Thymus</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus labeling PEX10 with 9671 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 7 Immunofluorescence Validation of PEX10 in Rat Testis</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PEX10 with 9671 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Documents

Further Information

Additional Names:
PEX10 Antibody: Peroxin-10, Peroxisomal biogenesis factor 10, Peroxisome assembly protein 10, RING finger protein 69, RNF69
Application Note:
WB: 4 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
Background:
PEX10 is implicated in the biogenesis of peroxisomes. Mutations in this gene result in phenotypes within the Zellweger spectrum of peroxisomal biogenesis disorders, ranging from neonatal adrenoleukodystrophy to Zellweger syndrome.
Background References:
  • Warren D.S. et al. Am. J. Hum. Genet. 63:347-359(1998)
  • Okumoto K. et al. Hum. Mol. Genet. 7:1399-1405(1998)
  • Ota T. et al. Nat. Genet. 36:40-45(2004)
Buffer:
PEX10 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
Homology:
Predicted species reactivity based on immunogen sequence: monkey (100%), horse (100%), gorilla (100%), chimpanzee (100%)
Immunogen:
Anti-PEX10 antibody (9671) was raised against a peptide corresponding to 10 amino acids near the carboxyl terminus of human PEX10.

The immunogen is located within last 50 amino acids of PEX10.
ISOFORMS:
Human PEX10 has 2 isoforms, including isoform 1 (326aa, 37.1kD) and isoform 2 (346aa, 39.2kD). Mouse PEX10 has 1 isoform (324aa, 37.2kD). 9671 can detect PEX10 in human and mouse.
NCBI Gene ID #:
5192
NCBI Official Symbol:
PEX10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 37kD

Observed: 37 kD
Protein Accession #:
O60683
Protein GI Number:
3914299
Purification:
PEX10 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
O60683
User NOte:
Optimal dilutions for each application to be determined by the researcher.