Tryptophan Hydroxylase Antibody / TPH1

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NSJ Bioreagents
Product Code: NSJ-F54902
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54902-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54902-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Tryptophan Hydroxylase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human HEK293 cell lysate with Tryptophan Hydroxylase antibody. Predicted molecular weight ~51 kDa.
2 / 4
IHC testing of FFPE human brain tissue with Tryptophan Hydroxylase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of human ThP1 cells with Cytokeratin 18 antibody (green) and DAPI nuclear stain (blue).
4 / 4
Flow cytometry testing of human WiDr cells with Tryptophan Hydroxylase antibody; Blue=isotype control, Green= Tryptophan Hydroxylase antibody.
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Western blot testing of human HEK293 cell lysate with Tryptophan Hydroxylase antibody. Predicted molecular weight ~51 kDa.
IHC testing of FFPE human brain tissue with Tryptophan Hydroxylase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human ThP1 cells with Cytokeratin 18 antibody (green) and DAPI nuclear stain (blue).
Flow cytometry testing of human WiDr cells with Tryptophan Hydroxylase antibody; Blue=isotype control, Green= Tryptophan Hydroxylase antibody.

Documents

Further Information

Application Details :
Immunofluorescence: 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500
Description:
TPH1 is a member of the aromatic amino acid hydroxylase family. This protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 35-62 from the human protein was used as the immunogen for the Tryptophan Hydroxylase antibody.
Limitation:
This Tryptophan Hydroxylase antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P17752