MT-ND5 Antibody / NADH-ubiquinone oxidoreductase chain 5

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NSJ Bioreagents
Product Code: NSJ-F54903
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54903-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54903-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the NADH-ubiquinone oxidoreductase chain 5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human liver tissue with NADH-ubiquinone oxidoreductase chain 5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
IHC testing of FFPE human brain tissue with NADH-ubiquinone oxidoreductase chain 5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Western blot testing of human 1) CCRF-CEM and 2) Jurkat cell lysate with NADH-ubiquinone oxidoreductase chain 5 antibody. Expected molecular weight: 65-70 kDa.
4 / 4
Flow cytometry testing of human CCRF-CEM cells with NADH-ubiquinone oxidoreductase chain 5 antibody; Blue=isotype control, Green= NADH-ubiquinone oxidoreductase chain 5 antibody.
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IHC testing of FFPE human liver tissue with NADH-ubiquinone oxidoreductase chain 5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human brain tissue with NADH-ubiquinone oxidoreductase chain 5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) CCRF-CEM and 2) Jurkat cell lysate with NADH-ubiquinone oxidoreductase chain 5 antibody. Expected molecular weight: 65-70 kDa.
Flow cytometry testing of human CCRF-CEM cells with NADH-ubiquinone oxidoreductase chain 5 antibody; Blue=isotype control, Green= NADH-ubiquinone oxidoreductase chain 5 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:100
Description:
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 544-570 from the human protein was used as the immunogen for the NADH-ubiquinone oxidoreductase chain 5 antibody.
Limitation:
This NADH-ubiquinone oxidoreductase chain 5 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P03915