FPGS Antibody / Folylpolyglutamate synthase

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NSJ Bioreagents
Product Code: NSJ-F54904
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54904-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54904-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the FPGS antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human placental tissue with FPGS antibody. Predicted molecular weight ~65 kDa.
2 / 4
Western blot testing of human 1) K562 and 2) A2058 cell lysate with FPGS antibody. Predicted molecular weight ~65 kDa.
3 / 4
IHC testing of FFPE human lung carcinoma tissue with FPGS antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human K562 cells with FPGS antibody; Blue=isotype control, Green= FPGS antibody.
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Western blot testing of human placental tissue with FPGS antibody. Predicted molecular weight ~65 kDa.
Western blot testing of human 1) K562 and 2) A2058 cell lysate with FPGS antibody. Predicted molecular weight ~65 kDa.
IHC testing of FFPE human lung carcinoma tissue with FPGS antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human K562 cells with FPGS antibody; Blue=isotype control, Green= FPGS antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Description:
FPGS is the folylpolyglutamate synthetase enzyme. This enzyme has a central role in establishing and maintaining both cytosolic and mitochondrial folylpolyglutamate concentrations and, therefore, is essential for folate homeostasis and the survival of proliferating cells. This enzyme catalyzes the ATP-dependent addition of glutamate moieties to folate and folate derivatives.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 304-330 from the human protein was used as the immunogen for the FPGS antibody.
Limitation:
This FPGS antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q05932