SORBS2 Antibody / ARGBP2

NSJ Bioreagents
Product Code: NSJ-F54906
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54906-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54906-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the SORBS2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human colon carcinoma tissue with SORBS2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of fixed and permeabilized human U-251 cells with SORBS2 antibody (green) and anti-Actin (red).
3 / 4
Western blot testing of human MDA-MB-231 cell lysate with SORBS2 antibody. Predicted molecular weight ~124 kDa.
4 / 4
Flow cytometry testing of fixed and permeabilized human MDA-MB-231 cells with SORBS2 antibody; Blue=isotype control, Green= SORBS2 antibody.

IHC testing of FFPE human colon carcinoma tissue with SORBS2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human U-251 cells with SORBS2 antibody (green) and anti-Actin (red).
Western blot testing of human MDA-MB-231 cell lysate with SORBS2 antibody. Predicted molecular weight ~124 kDa.
Flow cytometry testing of fixed and permeabilized human MDA-MB-231 cells with SORBS2 antibody; Blue=isotype control, Green= SORBS2 antibody.

Further Information

Application Details :
Western blot: 1:500-1:1000, Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE): 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells)
Description:
Arg and c-Abl represent the mammalian members of the Abelson family of non-receptor protein-tyrosine kinases. They interact with the Arg/Abl binding proteins via the SH3 domains present in the carboxy end of the latter group of proteins. ARGBP2 is the sorbin and SH3 domain containing 2 protein. It has three C-terminal SH3 domains and an N-terminal sorbin homology (SoHo) domain that interacts with lipid raft proteins. The subcellular localization of this protein in epithelial and cardiac muscle cells suggests that it functions as an adapter protein to assemble signaling complexes in stress fibers, and that it is a potential link between Abl family kinases and the actin cytoskeleton.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 175-204 from the human protein was used as the immunogen for the SORBS2 antibody.
Limitation:
This SORBS2 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
O94875