DCK Antibody / Deoxycytidine kinase

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NSJ Bioreagents
Product Code: NSJ-F54908
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54908-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54908-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Deoxycytidine kinase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of mouse intestine tissue lysate with Deoxycytidine kinase antibody. Predicted molecular weight ~31 kDa.
2 / 3
IHC testing of FFPE human brain tissue with Deoxycytidine kinase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 3
Immunofluorescent staining of human HeLa cells with Deoxycytidine kinase antibody (green) and DAPI nuclear stain (blue).
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Western blot testing of mouse intestine tissue lysate with Deoxycytidine kinase antibody. Predicted molecular weight ~31 kDa.
IHC testing of FFPE human brain tissue with Deoxycytidine kinase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HeLa cells with Deoxycytidine kinase antibody (green) and DAPI nuclear stain (blue).

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE): 1:10-1:50
Description:
Deoxycytidine kinase is responsible for the phosphorylation of several deoxyribonucleosides and their analogs. Deficiency of this enzyme activity is associated with resistance to antiviral and anticancer chemotherapeutic agents, whereas increased enzyme activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. It is the rate limiting enzyme in the activation of many important anticancer and retroviral drugs and its activity is often decreased in cells that are resistant to cytosine arabinoside.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 171-200 from the human protein was used as the immunogen for the Deoxycytidine kinase antibody.
Limitation:
This Deoxycytidine kinase antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
P27707