Pyruvate Kinase Antibody / PKM2

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NSJ Bioreagents
Product Code: NSJ-F54909
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54909-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54909-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Pyruvate Kinase PKM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human skin tissue with Pyruvate Kinase PKM antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human HeLa cell lysate with Pyruvate Kinase PKM antibody. Predicted molecular weight ~58 kDa.
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Western blot testing of human K562 cell lysate with Pyruvate Kinase PKM antibody. Predicted molecular weight ~58 kDa.
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Flow cytometry testing of human K562 cells with Pyruvate Kinase PKM antibody; Blue=isotype control, Green= Pyruvate Kinase PKM antibody.
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IHC testing of FFPE human skin tissue with Pyruvate Kinase PKM antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human HeLa cell lysate with Pyruvate Kinase PKM antibody. Predicted molecular weight ~58 kDa.
Western blot testing of human K562 cell lysate with Pyruvate Kinase PKM antibody. Predicted molecular weight ~58 kDa.
Flow cytometry testing of human K562 cells with Pyruvate Kinase PKM antibody; Blue=isotype control, Green= Pyruvate Kinase PKM antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Description:
PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 383-417 from the human protein was used as the immunogen for the Pyruvate Kinase PKM antibody.
Limitation:
This Pyruvate Kinase PKM antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P14618