MFAP5 Antibody / Microfibrillar-associated protein 5

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NSJ Bioreagents
Product Code: NSJ-F54919
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54919-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54919-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Microfibrillar-associated protein 5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human skeletal muscle tissue with Microfibrillar-associated protein 5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 3
Western blot testing of human K562 cell lysate with Microfibrillar-associated protein 5 antibody. Predicted molecular weight: 19-25 kDa depending on glycosylation level.
3 / 3
Flow cytometry testing of human K562 cells with Microfibrillar-associated protein 5 antibody; Blue=isotype control, Green= Microfibrillar-associated protein 5 antibody.
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IHC testing of FFPE human skeletal muscle tissue with Microfibrillar-associated protein 5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human K562 cell lysate with Microfibrillar-associated protein 5 antibody. Predicted molecular weight: 19-25 kDa depending on glycosylation level.
Flow cytometry testing of human K562 cells with Microfibrillar-associated protein 5 antibody; Blue=isotype control, Green= Microfibrillar-associated protein 5 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100
Description:
MFAP5 is a 25-kD microfibril-associated glycoprotein which is rich in serine and threonine residues. The protein lacks a hydrophobic carboxyl terminus and proline-, glutamine-, and tyrosine-rich regions, which are characteristics of a related 31-kDa microfibril-associated glycoprotein (MFAP2). The close similarity between these two proteins is confined to a central region of 60 aa where precise alignment of 7 cysteine residues occurs. The structural differences suggest that this protein has some functions that are distinct from those of MFAP2.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 133-160 from the human protein was used as the immunogen for the Microfibrillar-associated protein 5 antibody.
Limitation:
This Microfibrillar-associated protein 5 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
Q13361